ALOX15 ELISA Kit

Produktnummer ABIN2042937

Dieses Human ALOX15 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human ALOX15 zu quantifizieren.

Kurzübersicht für ALOX15 ELISA Kit (ABIN2042937)

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails ALOX15 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Marke: BioAssay™

Sensitivität: < 0.57 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Arachidonate-15-Lipoxygenase (ALOX15) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-15-Lipoxygenase (ALOX15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-15-Lipoxygenase (ALOX15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arachidonate-15-Lipoxygenase (ALOX15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Andere Bezeichnung: Arachidonate-15-Lipoxygenase (ALOX15)

Pathways: Regulation of Actin Filament Polymerization